Electrochemical Investigations on a Third Generation Biosensor for Determination of Hydrogen Peroxide Based on Immobilization of Myoglobin on a Novel Platinum Nanoparticle/Carbon Nanotube/Ionic Liquid/Nafion Composite

摘要

A novel composite film based on platinum nanoparticles (PtNPs), multi-walled carbon nanotubes(MWCNTs), room temperature ionic liquid (RTIL) and Nafion, is an effective matrix to immobilizemyoglobin (Mb). Platinum nanoparticles were deposited on MWCNTs and characterized by scanningelectron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) techniques. Directelectrochemistry and electrocatalysis of Mb were studied in detail using differential pulse voltammetry(DPV), cyclic voltammetry (CV) and chronoamperometry (CA) methods. Using CA, the mediator-freebiosensor response was linear with hydrogen peroxide (H2O2) concentration in the range of 0.5 to 210 with a detection limit of 0.1 M.