Nanomolar Quantification of Polydatin at Boron Doped Diamond Electrode. Application in Dietary Supplements

摘要

In this research we demonstrated detailed electrochemistry polydatin. Polydatin is a natural compoundwith expressed antioxidant activity. Electrochemical behavior of polydatin was tested in the potentialrange from -1.5 V to 2 V at unmodified boron doped diamond electrode in various pH of supportingelectrolyte. It has been noticed that polydatin provides two oxidation peaks, one at around 0.73 V andsecond at around 1.3 V. In the reverse scan no reduction peak was observed. Quantification of polydatinwas done based on first oxidation peak using square wave voltammetry. After optimization of themethod, linear working range from 1·10 -7 M to 7·10 -5 M was obtained, with limit of detection and limitof quantification of 6·10 -9 M and 2·10 -8 M, respectively. Negligible interferences effects were noticed.Developed method shows excellent accuracy and precision toward detection of polydatin. Also,developed method was used for quantification of polydatin in dietary supplements.