A simple and Novel Electroanalytical Method for Determination of Brain Serotonin Based on the MWNTs/Al₂O₃/chitosan SPE

摘要

In this work, a simple and novel method for determination of brain serotonin (5-HT) in depressionmice was proposed with modified screen-printed electrode based on MWNTs/Al2O3/chitosan. Themodified SPE displayed excellent electrocatalytic activity towards the oxidation of 5-HT in real braintissue samples. Square wave voltammetry (SWV) was employed to optimize the experimentalconditions. The proposed sensor showed voltammetric responses with high and good sensitivity for 5-HT in optimal conditions. The peak current of 5-HT was proportional to the concentration in the rangeof 0.01 μM to 1.0 μM with the detection limit 0.005 μM. Compared with the HPLC method, the newlydeveloped method also presented good results in the detection of brain 5-HT level, which made itpossible for helping depression desiease diagnosis. In conlusion, our experimental results stronglysuggested that the established SPE could be used to the fast depression determination of 5-HT, whichmight offer a potential minotor tool for in future.