Development of a Simple, Selective, Stable and Ultrasensitive Poly(safranineano NiO) Modified Carbon Paste Electrode for Selective Detection of Rutin in Buckwheat and Green Tea Samples

摘要

Present study demonstrates the construction of a new electrochemical sensor by electropolymerizationof safranine on the surface of modified nano NiO carbon paste electrode. Cyclic and differential pulsevoltammetric techniques were applied for the detection of rutin using 0.1 M phosphate buffer solution(pH 5.5) as supporting electrolyte. Specific variables including solution pH, analyte concentration andscan rates were optimized to validate the method. The peak currents were proportional with rutinconcentration between 1.61x10 ?8 M to 2.30x10 ?7 M. The assessed limit of detection and limit ofquantification values were found to be 0.54x10 -8 M and 1.61x10 ?8 M respectively. The modifiedpoly(safranineano NiO)CPE was successfully employed for the selective quantification of rutin inbuckwheat and green tea samples in presence of other interfering substances. The recoveries were foundconsistent between 96.5%-102.8% with the modified poly(safranineano NiO)CPE. Hence, theelectrochemical response of poly(safranineano NiO)CPE for the detection of rutin was greatlyenhanced after electropolymerization, which is 3 times higher than the nano NiO modified CPE and 6times higher than the bare CPE. Overall, the proposed poly(safranineano NiO)CPE has showedsuperior electrocatalytic activity and higher sensitivity for the selective detection of rutin in real samples.