Neural stem cells transplantation alleviate the hyperalgesia of spinal cord injured (SCI) associated with down-regulation of BDNF

摘要

Transplantation of neural stem cells (NSCs) in the injured spinal cord has been shown to improve functional outcome. However, the influence of NSCs transplantation on the sensory function and analgesic behaviors has not been elucidated yet. Here, we investigated whether transplanted NSCs would improve sensory function in rats subjected to complete cord transection (T10) and explore the underlying mechanism. The rats were divided into sham, SCT (spinal cord transection), and NSC implanted groups. NSCs (3 × 10sup6/sup/ml) were implanted into injury site at the day after operation. Mechanical (the hind paw test) and thermal (the tail-flick test) were measured at 5 weeks. Immunohistochemistry and RT-PCR were used to demonstrate that expression of Brain-derived neurotrophic factor (BDNF) in the superficial of the dorsal horn. Consequently, the tail-flick latencies and paw withdrawal thresholds in NSC implanted group exhibit a significant higher than SCT group (P < 0.05). RT-PCR demonstrate that mRNA expression of BDNF was down-regulated remarkably in NSC engrafted rats. The present findings suggest that NSC transplantation inhibits neuropathic pain associated with BDNF down-regulation.