Screening identification and characterization of lipase producing soil bacteria from upper dir and mardan khyber pakhtunkhwa, pakistan

摘要

Microbial lipases are one of the most important extracellular metabolites which have been the main focus of scientific research due to its huge biotechnological usage over the years. Although there are many sources of lipases but microbes are the best prod ucers. In this regards, the present work was conducted to isolate and screen out lipolytic bacteria from soil of Upper Dir and Mardan areas. Later on the lipolytic activity was observed through optimization of cultural physico - chemical parameters. Screenin g of lipolytic bacteria was achieved by inoculation of sample on selective medium that is tributyrine agar. Out of thirty (30) soil samples, Twenty one (21) were lipase producers. Fifteen (15) were gram positive and six (6) were gram negative. Bacillus spp were further explored for growth and lipase production optimization. Bacillus spp produce optimum growth at 37 o C and pH 7. Furthermore, the optimum enzyme production for Bacillus spp was observed at pH 7 temperature 37°C and at 48hours time duration.