Detection of Blactx-M gene on resistant salmonella enterica from a hospital in southeast Nigeria

摘要

Typhoid fever is said to be a systemic infection caused by Salmonella Enterica serotype typhi . A total of fifty stool samples were analyzed out of which twenty-five clinical isolates of Salmonella enterica serovars recovered between July-September, 2010 were investigated and screened using molecular methods for the presence of Blactx-M type gene. Polymerase Chain Reaction analysis showed that the four of the S.enterica encoding the CTX-M type gene were mostly Extended Spectrum β-lactamase producers which had no plasmids DNA. Phenotypic analysis reveals that the enzymes were active on Ceftazidime (64%) more than Cefotaxime (16%). Two folds serial dilution method reveals that Cefotaxime had increased Minimum Inhibitory Concentration (MIC) ranging from 1.56ug/ml- 12.5ug/ml depending on the isolates and the β -lactamase enzyme produced. The four Blactx-M genes detected on the adult patients were from the General Out Patients Department (GOPD) and National Health Insurance Scheme (NHIS) section of the hospital in Eastern Nigeria.