Telomere dysfunction-related serological markers in patients with type 2 diabetes; Correlation with methylene tetrahydrofolate reductase (C677T) gene polymorphism and diabetic complications

摘要

Recent studies have identified a set of serological markers for telomere dysfunction and DNA damage. In the present study, the levels of 2 serological markers of telomere dysfunction namely chitinase and N-acetylglucosaminidase (NAG) were studied in type2 diabetic mellitus (T2DM) patients. The possibility that genomic damage, accumulation of reactive oxygen species and shorter telomeres may be linked to the onset and progression of diabetes and it is complications A total of 38 patients with T2DM together with 15 healthy persons comparable in age and sex with patients were included, the serum samples were used for determination of chitinase, NAG and lipid peroxide (LPER) by colorimetric methods, and homocysteine by ELISA. methylene tetrahydrofolate reductase (MTHFR) C677T gene polymorphism was determined by polymerase chain reaction(PCR). Serological chitinase, NAG, LPER and homocysteine were significantly increased in T2DM compared with controls and correlated significantly with age. Moreover, in T2DM showed that the genotype frequencies were CC (36.48%), CT (39.47%) and TT (23.68%). Patients with mutant gene, CT, TT showed significantly elevated indices compared to CC type. Serological chitinase and NAG were the recent markers of telomere dysfunction and DNA damage were found to be markedly increased in T2DM.