Analysis of Phytochemical Components and Anti-microbial Activity of the Toxic Plant - Thevetia peruviana

摘要

Medicinal plants are widely distributed in the country. Among these, the toxic plant Thevetia peruviana also comes under the medicinal plants category and it has many medicinal uses. The leaves of Thevetia peruviana were extracted with ethanol and then it was purified. Proteins and phenols were confirmed by performing TLC (Thin layer chromatography). Glycosides were confirmed by Borntrager's test. The purified sample was used to analyze the phytochemical compounds by GC/MS (Gas chromatography/Mass spectroscopy). The amount of glycosides present in the leaf extract was quantified using Spectrophotometer. Antimicrobial was tested against Escherichia coli, Salmonela typhi, Staphylococcus, Vibrio cholera and Shigella . The result showed the presence of proteins, phenols and glycosides in the leaf extract. Eleven phytochemical components were obtained GC/MS.