The alteration of brain connectivity in frontal lobe epilepsy patients based on the alpha band analysis after vagal nerve stimulation

摘要

However, it is unclear how functional connections are stabilized over time to establish coherent representations of distinct environments during novel experiences. Neural responses to spatial novelty have been understudied because alternating between familiar and novel envi- ronments while simultaneously recording from many individual neurons that form ensembles in behaving animals is challenging. In this study, we utilized the flexibility of virtual reality (VR) and the temporal precision of in vivo multi-channel recordings to detect single unit and population activity during the first moments of nov- elty exposure in behaving mice. We trained wild-type mice to lickin specific locations on a virtual track while running on a linear tread- mill. We then recorded local field potentials and single-unit activity simultaneously in CA3 in animals exploring both familiar and novel environments in VR. We assessed changes in spiking activity of putatively classified interneurons and pyramidal cells in CA3 while animals explored both familiar and novel virtual environments. This study provides insights into how different hippocampal cell- types contribute to the emergence of stable spatial representations.