Antagonistic Activity of Selected Isolates of Fluorescent Pseudomonas Against Fusarium oxysporum f. sp. ciceri

摘要

The antagonistic activity against in vitro growth of Fusarium oxysporum f. sp. ciceri was determined for 90 isolates florescent Pseudomonas obtained from the rhizosphere and rhizosphere of healthy, partially wilted and completely wilted chickpea. Based on zone of inhibition, isolates were categorized into four categories, i.e. highly, moderately, least and non-antagonists. Maximum number of highly antagonistic isolates was obtained from rhizosphere and rhizoplane of healthy chickpea plants, whereas, maximum number of non-antagonistic isolates were obtained from completely wilted chickpea plants. Isolate H-Pf5 and C7R12 were selected for green house and field bioassays. Cell free culture filtrate of both the selected isolates was able to inhibit conidial germination of pathogen. Under green house and field conditions the isolates of fluorescent Pseudomonas , significantly enhanced seed germination, reduced disease incidence and promoted plant growth of chickpea as compared to control.