The Inhibition of Andrographolide ( Andrographis paniculata) on the Expression of Carbonic Anhydrase in LPS-Induced Human Leucocyte Cells

摘要

The aim of this work was to explore the bioactivity andrographolide, a major labdane diterpenoidal constituent of Andrographis paniculata , especially its influence on the expression of certain protein in LPS-induced human leucocyte cells. Human leucocyte cells were isolated from human whole blood and preincubated with andrographolide (25-200 μM) for 15 min. The preincubated cells were treated with lipopolysaccharide for 1 h afterwards. The method used in this work was SDS-PAGE , a polyacrylamide gel electrophoresis with a sodium dodecyl sulfate-containing buffer. Andrographolide inhibited the expression of certain protein which molecular weight was 36 kDa and identified as carbonic anhydrase. Since this enzyme produces and uses protons and bicarbonate ions, inhibition of this enzyme might lead to a decrease of acid production in the stomach and mild alkalosis.