Callus induction and organogenesis capacity from lamina and petiole explants of Anthurium andreanum Linden (Casino and Antadra)

摘要

This paper describes a rapid and efficient protocol to propagate Anthurium andreanum Linden cultivars Casino and Antadra in presence of IBA, NAA, 2,4-D, KIN and BA through callus induction and organogenesis. Segments of lamina and petiole (micro-cuttings or explants) were cultured in MS basal medium with different concentrations of NAA (0.0, 0.01, 0.1, 0.5, 1 and 2 mg/L) and BA (0.0, 0.5, 1, 2 and 3 mg/L) to produce callus. After 65 days, the most callus production was observed in medium containing 0.5 mg/L NAA + 3 mg/L BA in dark conditions. Production of callus in younger explants grown in dark was better than that the older explants grown in light conditions. The development of shoots and plantlets was initiated later from calluses. NAA (0.0, 0.005, 0.01 and 0.02 mg/L), 2,4-D (0.00, 0.05 and 0.1 mg/L), KIN (0.0 and 1.0 mg/ L) and BA (0.0 and 1.0 mg/L) were used for shoot proliferation. The best proliferation of shoots per callus (22.83 shoots per cm2 of callus) was observed on medium supplemented with 0.01 mg/L NAA + 1 mg/L BA after 8 weeks in a 16/8 h light and dark cycle under a photoperiod of 50 μmol/m2/s. Callus production and shoot proliferation were better in Antadra cv. than those of Casino. IBA (0.0, 0.5, 1.0 and 2.0 mg/L), NAA (0.0, 0.05, 0.1 and 0.25 mg/L) and KIN (0.0 and 0.2 mg/L) were applied for rooting of proliferated shoots. In root induction media, the largest number of root (11.50 roots per plantlets) was obtained on medium supplemented with 1 mg/L IBA + 0.2 mg/L KIN. Rooting was significantly higher in Casino cv. Regenerated plants were transferred to peat: perlite: sand (1:1:1) after hardening and they showed 96% of survival.