Partial polymerase gene sequence, phylogeny and RT-PCR diagnostic assay for Datura yellow vein nucleorhabdovirus

摘要

Partial (c. 0.25 and 1.1?kb) nucleotide sequences of the L polymerase gene of Datura yellow vein virus (DYVV) were obtained using two sets of degenerate oligonucleotide primers. This is the first sequence information for this virus. Phylogenetic analysis of the conserved L gene sequence with those of other plant rhabdoviruses showed that DYVV is most closely related to Sonchus yellow net virus and confirmed its taxonomic placement in the genus Nucleorhabdovirus. DYVV strains isolated from Datura stramonium and Thungergia alata, respectively, were 97.2?% identical in nucleotide sequence across a 251?nt region of the L gene. DYVV-specific primers were designed and a diagnostic RT-PCR assay developed and validated.