Molecular cloning and expression of 12-oxophytodienoic acid reductase gene from barley

摘要

The 12-oxophytodienoic acid reductases (OPRs) are flavin mononucleotide-dependent oxidoreductases that catalyse the reduction of double bonds in α,β-unsaturated aldehyde and ketones and some of them are part of the octadecanoid pathway leading to jasmonic acid biosynthesis. In this paper, a member of the OPR gene family (HvOPR1) was isolated from barley, the full length cDNA of HvOPR1 was 1298 bp containing a 1089 bp ORF encoding 362 amino acids. Bioinformatic analyses revealed that the deduced HvOPR1 has considerable homology with other plant OPRs. Phylogenetic analysis showed that HvOPR1 codes for OPR of subgroup I, containing enzymes which are not required for jasmonic acid biosynthesis. Expression of HvOPR1 during development and in response to abiotic stresses and hormonal treatments was analyzed by semi-quantitative reverse-transcription PCR. HvOPR1 expression was differentially regulated during primary leaf development and up-regulated in response to drought, hydrogen peroxide and wounding. Moreover, the accumulation of HvOPR1 mRNA was increased in response to jasmonic acid, salicylic acid and abscisic acid. The results suggested that HvOPR1 might play an important role during development and in response to abiotic stresses and signaling molecules.