Estimating the genetic purity in cytoplasmic male sterile (CMS) lines of Egyptian rice

摘要

Cytoplasmic male sterile (CMS) lines often get contaminated with cognate isonuclear maintainer lines during multiplication. The fingerprinting of rice hybrids and their respective parental lines and testing genetic purity of rice hybrids using genetics markers are tested in the present study. To develop a reliable polymerase chain reaction (PCR) assay for distinguishing CMS and maintainer lines, a recommended primer pair called drrcms was used. PCR was performed with drrcms marker using the template DNA from a CMS line (IR 70368A), its cognate isonuclear maintainer line (IR 70368B), and the hybrid. This marker could unambiguously distinguish CMS (or the hybrid) from maintainer line. The PCR-based DNA marker drrcms described helps to detect contamination of maintainer and other male fertile lines in seed lots of WA-CMS lines. Up to our knowledge, this is the first study reported drrcms marker used to amplify distinct fragments in Egyptian CMS and their cognate isonuclear maintainer lines.