EVALUATION OF IMMUNOMODULATORY ACTIVITY OF AQUEOUS EXTRACT OF A POLYHERBAL FORMULATION BY IN-VIVO METHOD

摘要

The objective of the present study was to evaluate the immunomodulatory activity of aqueous extract of polyherbal formulation (RA-1). The assessment of immunomodulatory activity was carried by testing the humoral (Haemagglutination antibody titer (HA) model & zinc sulphate turbidity test) and cell mediated immunity (Delayed type hypersensitivity reaction model) using aqueous extract of RA-1, at 200 & 400mg/kg/day, p.o.b.w. for 21 days in healthy albino rats.. RA-1 showed a significant (P<0.05) increase in both the primary and secondary HA titer values and increase in serum immunoglobulin levels when compared with control which indicates it’s stimulatory effect on humoral immunity. On other hand, in delayed hypersensitivity test, RA-1 significantly (P<0.01) potentiated the DTH response when comparing with control, indicates its triggering effect on cell mediated immunity. From the above results, it was concluded that the aqueous extract of RA-1 was safe at dose of 2000mg/kg. The active constituents like flavonoids, terpenoids & glycosides were found to be present in aqueous extract of RA-1 and it also has good stimulatory effect on both humoral and cell mediated immunity. This study comprises of safety, preliminary phytochemical screening and immunomodulatory activity of aqueous extract of RA-1.