ALTERATIONS INDUCED BY N-NITROSODIMETHYLAMINE AND ETHANOLIC ROOT EXTRACT OF OPERCULINA TURPETHUM IN SERUM LIPID PROFILE OF MALE ALBINO MICE

摘要

The present study investigated the protective effect of ethanolic ro ot extract of Operculina turpethum on serum lipid profile in male albino mice intoxicated with N-Nitrosodimethylamine (NDMA). N-nitrosodimethylamine is a potent hepatotoxin and carcinogen and has been reported to be found in the chloraminated drinking water from treatment plants and distribution systems. Operculina turpethum Linn is convolvulaceous plant, the roots are bitter, acrid, sweet, thermogenic, purgative, carminative, anthelmintic, expectorant, antipyretic, hepatic stimulant and effective against colic obesity and high lipid levels. Phytochemicals detected in the ethanolic extract are Flavonoids, Polyphenols, Alkaloids, Saponins, Enzymes, and Minerals. Adult male albino mice, treated with NDMA at a concentration of 10 mg/kg body weight and Operculina turpethum root extract was given orally in doses of 300 and 400 mg/kg body weight at 5 h after the administration of NDMA. NDMA treated mice showed a significant decrease in the levels of low density lipoprotein (LDL) in the serum of mice received OTE at a concentration 300mg/kg b.wt. Further, an increase in the level of high density lipoprotein (HDL) in serum of mice who received OTE (400 mg/kg b.wt.) after intoxication with NDMA and a significant decrease in the level of cholesterol and triglycerides of mice administrated with OTE at a concentration of 300mg/kg b.wt. was observed. Administration of ethanolic extract at a dose of 300 mg/kg bd.wt. significantly attenuated the alterations caused by the intoxication of NDMA when compared with the standard BHA and restored the levels of triglycerides in the serum of mice