STANDARDIZATION, DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF EMBELIN AND GALLIC ACID AS INDIVIDUAL AND IN COMBINATION IN AYURVEDIC CHURNA FORMULATION

摘要

Objectives: A new simple spectrophotometric method for simultaneous estimation of Embelin and Gallic Acid in Vidang, Harade and Vidangadi Churna was investigated. The λmax of Embelin and Gallic Acid were found to be 341 and 258 nm. Method: The graphical absorbance ratio method was used for simultaneous estimation of Embelin and Gallic acid in Vidangadi Churna, and the Isoabsorbtive point for simultaneous estimation of both the drug were found to be 307.5 nm. The method obeys Beers law in the concentration range from 2-12 μg/ml. The correlation coefficient was found to be 0.9973 for Embelin and 0.998 for Gallic Acid. The method was validated as per ICH guidelines. Results: The result of estimation of Embelin in Vidang, Gallic acid in Harade and both in Vidangadi Churna were found to be 1.05 percent, 1.04 percent, and 1.85. The proposed method was validated for linearity, accuracy and application for assay as per ICH guidelines. Conclusion: The study showed good reproducibility and recovery with percent RSD less than 2. So, the proposed method was found to be simple, specific, precise, and accurate. Hence it can be applied for simultaneous estimation of Embelin and Gallic Acid in various Ayurvedic Churna formulations.