Studying the Impact of Golgi Protein 73 Serving as a Candidate Biomarker in Early Diagnosis for Hepatocellular Carcinoma among Saudi Patients

摘要

Background: Due to the prevalence of Hepatocellular carcinoma (HCC) in Saudi Arabia, using new markers togive best diagnostic performance than alpha-feto protein (AFP) are important in early diagnosis. The aim of this workwas to compare the significance between serum and mRNA Golgi glypican73 (GP-73) as newly identified diagnosticand prognostic markers for HCC among Saudi patients. Materials and Methods: A total of 300 subjects were dividedinto: 250 blood samples where 145 samples from HCC, 105 samples from chronic liver cirrhosis (CLC) and 50 normalcontrols were investigated for serum GP73 (sGP73) by ELISA. GP-73 mRNA from peripheral blood mononuclear cellswas amplified by RT-PCR. The sensitivity and specificity of both techniques was compared. Results: Serum Golgiglypican 73 was significantly higher in HCC group compared to cirrhotic and normal controls (p0.001). Sensitivityand specificity were 95% for sGP-73, 100% and 90% for Golgi glypican 73 mRNA. The combination of sensitivitybetween AFP and sGP73 was 80% and 95% respectively. Conclusion: Both serum Golgi glypican-73 and GP-73Mrnaare good diagnostic biomarkers for early detection of HCC in Saudi patients. RT-PCR is more accurate and sensitive(100%) than ELISA (95%) in detecting Golgi glypican 73.