Tylophorine Abrogates G2/M Arrest Induced by Doxorubicine and Promotes Increased Apoptosis in T47D Breast Cancer Cells

摘要

Background: The effects of tylophorine, a natural alkaloid found in Tylophora indica, administered as a singlecompound or in combination with doxorubicin on cell cycling and apoptosis were assessed in T47D breast cancer cells,selected as a model system for breast cancer. Methods: Cell cycle distribution and apoptosis were examined by flowcytometry. Caspase 3 and 9 expression was determined by immunocytochemistry.Result: We found that tylophorine didnot significantly influence the cell cycle distribution of T47D cells. However, the alkaloid did prevent accumulation ofcells in the G2/M phase. In addition, tylophorine increased the number of apoptotic cells. Expression of proapoptoticproteins (caspases 3 and 9) was up-regulated upon administration of tyloporine alone or in combination with doxorubicin.Conclusions: Tylophorine alone or in combination with doxorubicin induced apoptosis in T47D breast cancer cellsthrough modulation of the cell cycle and affecting the expression of caspases 3 and 9.