Effect of Preparation and Storage Methods of Platelet Products on Complement Activity

摘要

Platelet transfusion is considered a vital therapeutic approach for several diseases. However, like other blood products, it can cause transfusion reactions mainly due to various plasma proteins. With regard to provision of platelet products by a variety of methods including random donors and platelet apheresis, the risk of adverse reactions is assumed to be different in products produced by different methods. The aim of this study was to evaluate the difference in the level of C3 and its derivatives (C3a and C3b anaphylatoxins) as a plasma protein causing reactions in random and platelet apheresis methods. The study was conducted on healthy volunteer blood donors referred to Shiraz Blood Transfusion Organization. Platelet products were prepared by the two mentioned methods based on standard operating methods approved by the Iranian Blood Transfusion Organization. The C3 level was evaluated in three time points using immunoturbidimetric test: immediately after processing, one day and three days later. The data were analyzed using SPSS 16 software in terms of student t test and Pearson correlation. The p<0.05 was considered statistically significant. The C3 level was in maximum level in both method of platelet preparation immediately after preparation and was in minimum in the first day. In addition, statistically significant difference was observed between C3 level in each point of measurement: immediately after preparation (p = 00), the first day (p = 00) and on the third day (p = 0.45). A significant correlation was also observed between C3 level and type of platelet storage bag. Our findings show that the method of platelet preparation and storage plays an important role in the level of accumulated C3 in these products. The C3 concentration, the resulting complement activity and accumulation of anaphylatoxin factors was higher in this product. Therefore, further investigation might prove which method of platelet preparation bears a lower risk of reactions related to the injection of this product.