Cellulase Production by Bacillus subtilis isolated from Cow Dung

摘要

Cellulases are the enzymes hydrolyzing cellulosic biomass and are produced by the microorganisms that grown over cellulosic matters. Bacterial cellulases possess more advantages when compared to the cellulases from other sources. Cellulase producing bacteria was isolated from Cow dung. The organism was identified using 16 SrDNA sequencing and BLAST search. Cellulase was produced and the culture conditions like temperature, pH, and Incubation time and medium components like Carbon sources, nitrogen sources and role of natural substrates were optimized. The enzyme was further purified using ethanol precipitation and chromatography. Cellulase was then characterized using SDS-PAGE analysis and Zymographic Studies. The application of Cellulase in Biostoning was then analyzed.