Anti-implantation effect of chlorpyrifos in Swiss albino mice

摘要

Experiment was conducted to evaluate the anti-implantation effect of sublethal doses ofchlorpyrifos (CPF) in Swiss albino mice. Thirty five female Swiss albino mice were randomlydivided into 5 groups of 7 mice in each group served as the experimental subjects for this study.Each female mouse was cohabited with a proven fertile male in a cage for the purpose of mating.The female mice were exposed orally to different doses of CPF starting from the day ofconfirmation of mating termed gestation day (GD) 1 up to GD5. Mice in group 1 served as thecontrol and were administered vegetable oil, while those in group 2 were dosed with CPF at 10.6mg/kg (~ 10% LD50). Mice in groups 3, 4 and 5 were exposed to CPF at 15.9 mg/kg (~15% LD50),21.2 mg/kg (~20% LD50) and 26.6 mg/kg (~25% LD50), respectively. At GD8 the mice weresacrificed and the ovaries and the uterine horns examined for the number of corpora lutea andimplantation, respectively. The number of pregnant animals and the percentage preimplantationloss in each group were evaluated. The result showed a dose-dependent increase inpreimplantation loss in all the groups exposed to CPF.