Screening of some marine-derived fungal isolates for lignin degrading enzymes (LDEs) production

摘要

A study was carried out to establish the lignin-degrading enzymes (LDEs) activity of a largenumber of diverse marine ascomycetes. Eighty eight fungal isolates obtained from different algae,sea grasses and decaying wood samples collected from Abou-keer, Alexanderia, Egypt, werescreened for the presence of laccase (Lac), lignin-peroxidase (LiP) and manganese-dependentperoxidase (MnP) activities. Results obtained from both qualitative and quantitative assayshowed that the marine fungal isolate Trematosphaeria mangrovei measured the highest zonediameter and colony diameter in agar plate screening test with guaiacol and showed a finalspecific activity of 82.51 U/mg protein with higher laccase activity 14.03 U/ml when grown in lownitrogen (LN) medium with half-strength sea water, initial pH 4.5 for 14 days. The other twoligninolytic activities could not be detected. It was recommended that the marine fungal isolateTrematosphaeria mangrovei was the most promising one for laccase enzyme production.