Cloning and Expression Analysis of RrG-Beta1 Gene Related to Anthocyanin Biosynthesis in &i&Rosa rugose&/i&

摘要

As an important signal transduction protein, G protein beta subunit gene encoded by oligonucleotides plays an important role in many physiological, biochemical and environmental stresses in plants. In order to understand the action mode of G protein beta subunit gene, this paper cloned a Wd 40 gene related to G protein beta subunit gene, named RrG-beta1, based on the R. rugose —transcriptome data, using Rosa rugose “Zi zhi” as experimental materials. The full length of cDNA of the gene was obtained by RT-PCR and RACE methods. The total length of this gene is 981 bp, and it encodes 326 amino acids. After bioinformatics analysis, the molecular formula C_(1601)H_(2520)N_(450)O_(486)S_(11) was predicted; the relative molecular weight was 36,201.00 Da; the theoretical isoelectric point PI value was 6.71; and its instability index was 30.44. The total average hydrophobic index was -0.847. In the secondary structure of RrG-beta1 protein, there are 17 α -helix, 131 Random coil, and 141 extended peptide chain. Gene Bank Blast results showed that the amino acid sequence encoded by RrG-beta1 was more than 90% homologous with the beta-like protein of Rosa chinensis , Fragaria , Malus , Pyrus , Prunus , Arabidopsis and tobacco , so it can be inferred that the RrG-beta1 Gene is guanine nucleotide-binding protein subunit beta-like protein. Fluorescence quantitative expression analysis of RrG-beta1 protein decreased with the development of flower color, and it was speculated that it could exert negative regulation effect on flower color. The leaf expression was highest in the tissue part, so it was inferred that the signal was transmitted through the stoma on the leaf.