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In Vitro Micropropagation of a Valuable Medicinal Plant, Plectranthus amboinicus

机译:珍贵药用植物凤尾兰的体外微繁殖

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The effect of the plant growth regulators benzyl amino purine (BAP), 1-naphthaleneacetic acid (NAA) and kinetin (KIN) on in vitro shoot induction and proliferation of Plectranthus amboinicus was examined. Explants obtained from lateral shoots and apical shoots of P. amboinicus were inoculated on Murashige and Skoog (MS) culture medium supplemented with different concentrations of BAP, NAA and KIN. When the effect of each growth regulator was considered singly, the highest rate of shoot induction (80% of explants producing shoots) and highest number of shoots produced (2.4 shoots per explant) were obtained from lateral shoot explants cultured on MS media supplemented with 3.0 mg/L BAP within 6 - 7 weeks. Better results were obtained using MS medium supplemented with 1 mg/L BAP + 5 mg/L NAA. Shoot proliferation rose to 85%, while 5.7 shoots per explants were recorded. Among the different media tested for rooting, MS medium supplemented with 1.0 mg/L IBA was the most effective for root induction. The quality of the roots obtained was better than that obtained using MS media supplemented with NAA or IAA.
机译:考察了植物生长调节剂苄基氨基嘌呤(BAP),1-萘乙酸(NAA)和激动素(KIN)对蓝花侧柏体外芽诱导和增殖的影响。在补充了不同浓度的BAP,NAA和KIN的Murashige和Skoog(MS)培养基上接种从竹节单胞菌的侧芽和根尖获得的外植体。当单独考虑每种生长调节剂的作用时,从在补充了MS的MS培养基上培养的侧芽外植体可获得最高的芽诱导率(80%的外植体产生芽)和最高的芽数(每个外植体2.4个芽)。 6-7周内BAP为3.0 mg / L。使用补充了1 mg / L BAP + 5 mg / L NAA的MS培养基可获得更好的结果。枝条增殖增至85%,同时记录每外植体5.7条。在测试生根的不同培养基中,补充有1.0 mg / L IBA的MS培养基对根的诱导最有效。所获得的根的质量优于使用添加了NAA或IAA的MS培养基获得的根。

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