Genetic Diversity of Wild Soybeans from Some Regions of Southern China Based on SSR and SRAP Markers

摘要

There are rich annual wild soybean (Glycine soja) resources in Southern China, which are the progenitor of cultivated soybean. To evaluate the genetic diversity and differentiation of G. soja in Southern China, we analyzed allelic profiles of 141 annual wild soybean accessions from Southern China and 8 core wild soybean accessions fromNorthern Chinaby using 41 simple sequence repeat (SSR) markers and 18 Sequence-related amplified polymorphism (SRAP) primer combinations. The 41 SSR markers produced a total of 421 alleles (10.27 per locus) with a mean of gene diversity of 0.825 (Simpson index) and 1.987 (Shannon-weaver index). The 18 SRAP primer combinations detected a total of 90 polymorphism bands (5 per primer combination) with a mean of gene diversity of 0.918 (Shannon-weaver index). SSR and SRAP markers detected 43 and 5 rare alleles in 149 wild soybeans, respectively. The wild soybeans from Fujian province showed the highest genetic diversity with Shannon-weaver index of 1.837 (by SSR) and 0.803 (by SRAP), and the highest allelic richness with an average of 8.8 alleles per locus and the most number of rare alleles of 0.68 per locus based on SSR data. An analysis of Molecular Variance (AMOVA) analysis showed that significant variance did exist amongHunan,Fujian, Guangxi andNorthern Chinasubpopulations based on SSR and SRAP data. The unweighted pair-group method of the arithmetic average (UPGMA) cluster analysis indicated that the wild soybeans fromFujianprovince occurred in different clusters based on both SSR and SRAP data. The above results indicated thatFujianprovince could be the major center of genetic diversity for annual wild soybean inSouthern China. In addition, Mantle test showed there was a weak positive linear correlation (r = 0.25) between SSR and SRAP analysis in the study.