Genome-Wide Identification and Characterization of the Dof Transcription Factor Gene Family in Phaseolus vulgaris L.

摘要

The Dof (DNA-binding with one finger) proteins are a class of plant-specific transcription factors that can trigger several processes involved in plant growth and development, as well as in stress responses. Here, we performed a systematic bioinformatics analysis to characterize all Dof genes in common bean, which included analysis of the genome sequence, conserved protein domains, chromosomal locations, subcellular locations, phylogenetic relationships, gene duplications, and gene expression profiles in different tissues. Bioinformatics analysis revealed 36 putative genes related to style="font-family:Verdana;">PvDof style="font-family:Verdana;"> that were classified into seven subfamilies (A, B1, B2, C1, C2, D1, and, D2) by comparative phylogenetic analysis. Based on our genome duplication analysis, a total of 36 genes were found to be distributed on all 11 chromosomes, and they expanded through gene duplication in tandem, suggesti style="font-family:Verdana;">ng style="font-family:""> style="font-family:Verdana;"> the involvement of segmental duplication events in the evolutionary process. Synteny events and phylogenetic comparisons of the Dof proteins of common bean with those of style="font-family:Verdana;">A. style="font-family:Verdana;">thaliana style="font-family:Verdana;">, style="font-family:Verdana;">O. style="font-family:Verdana;">sativa style="font-family:Verdana;">, and style="font-family:Verdana;">G. style="font-family:Verdana;">max style="font-family:Verdana;"> L. led to the identification of several orthologous and paralogous genes, which provided further insight into the diversity of the evolutionary characteristics of genes of this family in other plant species. Expression profiles revealed that most of the style="font-family:Verdana;">PvDof style="font-family:Verdana;"> genes were expressed in different tissues, indicating that style="font-family:Verdana;">PvDof style="font-family:Verdana;"> genes may be involved in various physiological functions during plant development. The results of this study provide additional information and potential biotechnological resources for further understanding the molecular basis of this gene family and consequently improvement of common bean crops.