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Identification of Molecular Markers Linked to Leaf Rust Resistance Genes in Wheat and Their Detection in the Local Near-Isogenic Line

机译:小麦抗叶锈基因相关分子标记的鉴定及其在近等基因系中的检测

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摘要

Sixty-five random amplified polymorphic DNA (RAPD) primers were used for the detection of polymorphism among recipient and donor parents and their isogenic lines linked to leaf rust resistance genes, Lr9 and the resistant gene in kharchia local mutant KLM4-3B. Three primers showed polymorphism among recurrent parent, donor parent and isogenic lines.
机译:65个随机扩增多态性DNA(RAPD)引物用于检测受体和供体亲本之间的多态性,以及与kharchia本地突变体KLM4-3B中的叶锈病抗性基因Lr9和抗性基因相关的等基因系。三种引物在轮回亲本,供体亲本和同基因系之间表现出多态性。

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