Investigation of the Gene Expression Levels of 5 α-reductase, VEGF and IL-1α in HaCaT Cells after the Application of a Botanical Extract

摘要

Background:Hair loss also known as alopecia is a common dermatological problem both for men and women all over the world. Deficiency in nutrition of the hair due to decrease in the micro-circulation in the scalp, or aging of the follicle cells due to lack of care are counted as significant causal reasons for hair loss. Treatment methods by using natural products such as plants and their derivatives are of great interest. In our study, HaCaT human keratinocyte cells were used as a model to demonstrate the effect of a botanical extract resulting from the specific plants of a unique Northeastern region of Turkey (Anzer) on gene expression of the cells.Material and Methods:Cell proliferation assay was performed by XTT reagent. After determination of non-cytotoxic concentration, cells were treated with plant mix and incubated for five days. RNA isolations were carried out from both non-treated and treated cell groups by using Tri-reagent. Gene expression of VEGF, IL-1a and 5-alpha reductase was determined by qPCR analysis.Results:The results demonstrated that Plant extract used in the current study revealed anti-inflammatory and anti-androgenic properties by downregulating IL-1α a and 5-α-reductase gene expressions, respectively. Furthermore, it led to upregulate VEGF gene expression which is a significant growth promoting factor for HaCaT cells.Conclusion: This plant mix seems to be a promosing candidate for preventing hair loss due to its anti-inflammatory, anti-androgenic and hair promoting properties.