In vitro and in vivo anthelmintic activity of pumpkin seeds and pomegranate peels extracts against Ascaridia galli

摘要

Pumpkin seeds (Cucurbita pepo) and Pomegranate peel (Punica granatum) have anthelmintic properties. The aim of this study was to compare the anthelmintic efficacy of pumpkin seeds ethanolic extract and pomegranate peel aqueous extract againstAscaridia galli in vitroandin vivoin Baladi chicks. On adult worms, the extracts of the two herbs were comparedin vitroat concentrations of 25, 50, and 75?mg/ml with fenbendazole at a concentration of 5?mg/ml. Chicks were infected withAscaridia gallieggs containing second stage larva and treated with 2000?mg/kg of each of the extracts compared with 100?mg/kg fenbendazole.In vitro, all concentrations of pumpkin seed extract and the concentration of 75?mg/ml pomegranate peel extract exhibited a nearly similar effect to fenbendazole.In vivo, the mortality rate of the worms extracted from the 2000?mg/kg pumpkin seeds extract-treated chicken was non-significantly different from that of fenbendazole for 48?h. While pomegranate peels extract exhibited a lower lethal effect than fenbendazole. The anthelmintic efficacy was dependent on time and concentration. The study presented the anthelmintic efficacy of the pumpkin seeds and pomegranate peel extracts onAscaridia galli.Pumpkin seed extract was more effective than pomegranate peel extract. Future studies to determine the optimal dose to maximize their effectiveness especially for pumpkin seeds as anthelmintic therapeutic are required.