Study of somatic embryogenesis in leaf explants of sapindus emarginatus vahl

摘要

Plant regeneration through Somatic embryogenesis has several advantages over other routes to in vitro plant production and appears that most promising area of research for large scale production and rapid plant propagation. Sapindus emarginatus vahl commonly known as soap nut tree wherein the nut shell contains saponin, which acts like soap when comes in contact with water. It has very high economic and medicinal values in saponin industry and is also being used for important medicinal properties. The present study is aimed on somatic embryogenesis from leaf explants of Sapindus emarginatus Vahl employing auxin such as 2, 4-D along with cytokinin BAP/Kn. The present protocol is the first ever reported of its kind and is unique in itself as the latex poses a threat for tissue culture.