Comparison of Escherichia coli KO11 and Saccharomyces cerevisiae ATCC 96581 in fermenting Pinus patula hydrolysate pretreated at different steam explosion severity

摘要

Bioconversion of wood residues to biofuels such as ethanol is one of the feasible initiatives towards production of renewable energy. This work compares the effectiveness of?Escherichia coli?KO11 and?Saccharomyces cerevisiae?ATCC 96581 in fermenting?Pinus patula?(pine) hydrolysate pretreated by acid-catalyzed steam explosion. The results show no significant difference in terms of the ethanol yield when?E. coli?KO11 and?S. cerevisiae?ATCC 96581 were used. The maximum ethanol concentration obtained in test tubes fermentation were 18.30 and 19.41 g/l for?E. coliKO11 and?S. cerevisiae?ATCC 96581, respectively, from samples pretreated at 225°C/5 min. The ethanol yields obtained using bioreactors for samples pretreated at 225°C, 0.5% SO2, and 5 min, were 21.30 and 19.63 g/l for?E. coli?and?S.?cerevisiae, respectively. Overall, ethanol yields were higher in bioreactors than in test tubes for both strains. In comparison,?S. cerevisiae?consumed the substrate faster than?E. coli, thus making?S. cerevisiae?the most preferred strain in fermentation of hydrolysates from steam pretreated?P. patula.