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首页> 外文期刊>African Journal of Biotechnology >Genetic variability of Indian yaks using random amplified polymorphic DNA markers
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Genetic variability of Indian yaks using random amplified polymorphic DNA markers

机译:印度random牛的遗传变异使用随机扩增的多态性DNA标记

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摘要

Indian yaks are categorized into common yaks, bisonian yaks and bareback yaks.Genetic similarities and divergence among them was analyzed by random amplified polymorphic DNA (RAPD) technique using ten decamer oligonuclotide primers. Only five primers (ILO 526, OPAV 15, ILO 1127, ILO 1065 and ILO 876) out of the ten primers tried produced consistant polymorphic fingerprints. Of the 76 fingerprints produced, 49 were present in all types, 21 were individual specific and 6 were polymorphic for different types. The pair wise comparison studied for different primers indicated the average bands sharing ranged from 78% in bareback to 88% in common type. There was no significant difference (P ≤ 0.05%) between the mean average percentage (MAPD) values observed between Indian yaks.
机译:印度牛分为普通牛,波黑牛牛和无鞍牛。使用十种十聚体寡核苷酸引物,通过随机扩增多态性DNA(RAPD)技术分析了它们之间的遗传相似性和差异性。在尝试的十种引物中,只有五种引物(ILO 526,OPAV 15,ILO 1127,ILO 1065和ILO 876)产生一致的多态指纹图谱。在产生的76个指纹中,所有类型都存在49个指纹,其中21个是个体特异性的,6个对于不同类型是多态的。对不同引物进行的成对比较研究表明,平均谱带共享范围从无鞍的78%到普通类型的88%。印度Indian牛之间的平均平均百分率(MAPD)值之间无显着差异(P≤0.05%)。

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