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首页> 外文期刊>African Journal of Biotechnology >Study of simple sequence repeat (SSR) polymorphism for biotic stress resistance in elite rice variety JGL 1798
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Study of simple sequence repeat (SSR) polymorphism for biotic stress resistance in elite rice variety JGL 1798

机译:简单重复序列(SSR)多态性对水稻优良品种JGL 1798抗生物逆性的研究

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To provide ready to use markers for back ground selection in marker assisted breeding of rice, we used GPP 2 as donor parent for?xa13, Xa21,?Gm4 resistance to bacterial blight, gall midge and NLR 145 as another donor parent for?Pi-kh?gene resistance to blast and JGL 1798 as recurrent parent was investigated using 128simple sequence repeat (SSR)?primers covered on chromosome number 1-12.? The results reveal that 36 HRM primers showed distinct polymorphism among the donor and recurrent parents studied indicating the robust nature of microsatellites in revealing polymorphism. Based on this study, the large range of similarity values for related cultivars using microsatellites provides greater confidence for the assessment of simple sequence repeats (SSR) polymorphism.
机译:为了提供可用于标记辅助水稻育种的背景选择的标记,我们使用GPP 2作为Δxa13,Xa21,ΔGm4对白叶枯病,胆mid的抗性的供体,以及NLR 145作为ΔPi-的另一种供体。利用128号简单重复序列(SSR)?引物(覆盖在第1-12号染色体上)研究了kh?基因对blast和JGL 1798作为轮回亲本的抗性。结果显示36种HRM引物在供体和轮回亲本之间显示出明显的多态性,表明微卫星在揭示多态性方面具有鲁棒性。根据这项研究,使用微卫星的相关品种的相似性值范围较大,可为评估简单序列重复(SSR)多态性提供更大的信心。

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