Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007

摘要

Samples from infected domestic pigs associated with an outbreak of?African swine fever (ASF) in three districts of central Uganda in 2007 were confirmed as being infected with African swine fever virus (ASFV) using a?P72?gene-based?polymerase chain reaction amplification (PCR)?assay combined with restriction analysis. None of the sera collected from pigs with clinical symptoms were positive using the OIE serological prescribed tests. However, seven haemadsorbing viruses were isolated in macrophage culture and genotyped by partial?p72?and full length?p54-gene sequencing. Four of these viruses were isolated directly from serum samples. All the viruses were classified within the domestic-pig cycle-associated?p72 and p54genotype IX which also includes viruses responsible for ASF outbreaks in Kenya in 2006 and 2007 and Uganda in 2003. To define virus relationships at higher resolution, typing was performed by analysis of tetrameric amino acid repeat regions within the central variable region (CVR) of the?B602L?gene. Ugandan isolates sequences exhibited 100% identity to viruses isolated from outbreaks in Kenya in 2007. The identity was greater than the viruses obtained from an earlier outbreak in Kenya in 2006. This provides further evidence that genetically similar ASFV virus within?p72?Genotype IX may be circulating between Kenya and Uganda.