Analgesic and anti-inflammatory effects of Crinum asiaticum leaf alcoholic extract in animal models

摘要

This study investigated the analgesic and anti-inflammatory effects of?Crinum asiaticum?(Amaryllidaceae)?leaf ethanolic extract.?Analgesic effect?was?investigated in acetic acid induced writhing model and formalin induced licking model in?swiss albino mice. Anti-inflammatory effect?was?conducted in?carrageenan-induced?paw edema model of?albino?rat.?Data were analyzed by one-way analysis of variance (ANOVA) followed by?post hoc?multiple comparison test.?In analgesic study,?C.?asiaticumextract inhibited 42.34±3.20% of acetic acid induced pain at higher dose?of?2.0 g/kg body weight.?The effect was statistically significant (p<0.001) compared to?the?positive control, diclofenac sodium (10 mg/kg).?The extract reduced the formalin induced pain22.60±1.39% in early phase and 27.11±0.87% in late phase?at the same dose?of?2.0 g/kg and the reductions were significant (p<0.01) compared to?the?positive control morphine (0.5 mg/kg). In?a time-dependent inhibition of carrageenan-induced paw edema model, the extract promoted the inhibitions of paw edema 51.60±2.50% at the 1st?h and 40.80±0.52% at the 4th?h of administration. These inhibitions were also significant (p<0.01) in comparison to those promoted by diclofenac sodium. No mortality was observed in acute toxicity test. The study concludes that?C. asiaticumleaf extract has potential analgesic and anti-inflammatory effects to be recorded as plant-derived complementary medicine.?Key?words:?Crinum asiaticum,?anti-inflammatory,?analgesic, Carrageenan,?formalin.