Melanoidin decolorization mechanism and some properties of Issatchenkia orientalis No.SF9-246

摘要

Issatchenkia orientalis?No. SF9-246 cell showed both melanoidin (MP) adsorption and degradation abilities and its MP-adsorption yield was reduced by acclimatization with medium containing MP. The living cell showed the MP-adsorption ability of 15%; higher than the autoclaved cell (dead cell). The MP-adsorption ability of the deteriorated cell (MP-adsorbed cell) was recovered by washing with 0.1 mol L-1?H2SO4?solution. Un-expectantly, the MP-adsorption capacity of the deteriorated cell was about 100 to 150% increased after washing with 0.1 mol L-1?H2SO4?solution. Also, the strain showed the highest biomass production yield of 43.6±2.5 g L-1?in the medium containing MP within 7 days cultivation and the crude protein content of the biomass was 36.38±1.12%. In addition, the strain produced intracellular glutathione?S-transferase enzymes types Y-1 and Y-2 (GST Y-1 and GST Y-2) and GST Y-1 production was induced by MP.