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TOOLS AND METHODS FOR GENOME EDITING ISSATCHENKIA ORIENTALIS AND OTHER INDUSTRIALLY USEFUL YEAST
TOOLS AND METHODS FOR GENOME EDITING ISSATCHENKIA ORIENTALIS AND OTHER INDUSTRIALLY USEFUL YEAST
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机译:基因组编辑东方原核酵母和其他工业用酵母的工具和方法
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摘要
The present description relates to genetic tools and methods to facilitate transformation and genetic engineering of industrially-useful yeast/fungal species, such as Issatchenkia orientalis, for which a robust set of genetic tools such as stably inherited and maintained plasmids and functional control sequences is presently lacking. Thus, the present description relates to autonomously replicating sequences (ARSs), RNA polymerase II/III promoters, RNA polymerase II/III terminators, expression cassettes, and vectors comprising same are described herein, as well as uses and methods relating to same, which are functional in I. orientalis. 展开▼