Inter-simple sequence repeat (ISSR) markers in the evaluation of genetic polymorphism of Egyptian Capsicum L. hybrids

摘要

DNA samples of six hybrids of?Capsicum?annuum?and?Capsicum frutescens?were analyzed with ten inter-simple sequence repeat (ISSR) primers, which produced 52 polymorphic bands out of 87 bands with polymorphism average of 60%. ISSR patterns scored five distinguishable species-specific bands; two for?C. frutescens?and three for?C. annuum?and 16 unique bands for hybrids individually that were considered as molecular markers for Egyptian hybrids. The genetic dendrogram among the materials constructed by the unweighted pair-group method with arithmetic average based on the Dice coefficient showed that the six hybrids can be separated into two groups with genetic distance at 0.25. The first cluster included hybrids of?C. frutescens?and the other grouped those? of?C. annuum?indicating narrow genetic base among the tested hybrids in both species. This study revealed considerable genetic diversity in the different?Capsicum?hybrids for conservation of genetic resources and efficient crop breeding programs.