Assessment of biodiversity based on morphological characteristics and RAPD markers among genotypes of wild rose species

摘要

Conservation and utilization of the native plant resources is essential for long termsustainability of biodiversity. Wild native resources are adapted to specific and diverse?environmental conditions and therefore, these adaptive features can be introduced into?modern cultivars either through conventional breeding or advanced molecular genetic?techniques. Understanding the genetic make up of the wildly growing plant?species and of target desirable genes is a prerequisite for this purpose. Five wild rose (Rosa?L.) genotypes were collected from different locations in northern hilly areas of?Pakistan for this?study. Different morphological characteristics and PCR based?random amplified polymorphic DNA (RAPD) technique was used to find out the diversity and relationship among the genotypes.On morphological basis,?Rosa?webbiana?collected from Muree and Nathia gali showed?maximum (83%) similarity, whereas on DNA pattern basis,?Rosa?brunoniicollected from?Bansra gali and Sunny bank showed maximum (72%) similarity, while?R. webbiana?showed maximum diversity among all the species.