Optimization of microsatellite DNA Gelred fluorescence imaging technology

摘要

Gelred fluorescent dye has broader prospects of application in DNA experiment because of its high sensitivity, security and stability. In order to explore the best microsatellite DNA Gelred imaging technology, this study compared its dosage by using three methods; precasting gels method (PG), staining sample method (SS) and immersion gels method (IG). The results show that agarose gel electrophoresis (AGE) fluorescence imaging technology can use the first method (PG) and the concentration of Gelred was 1X, because of the best banding and easy operation. The polyacrylamide gel electrophoresis (PAGE) can use the third method (IG), for the advantages of clear and bright image, saving dye and easily redying to image. The orthogonal test showed that the parameters of IG method were: the concentration of Gelred was 2X, that of sodium chloride was 10% and immersion time was 40 min. The optimization of microsatellite DNA Gelred fluorescence imaging technology would lay a technical foundation in DNA banding related experiments.?Key word:?Agarose gel electrophoresis (AGE),?polyacrylamide gel electrophoresis (PAGE),?fluorescence imaging technology of Gelred,?simple sequence repeat (SSR).