Determination of optimum dose and frequency of application of free-living diazotrophs (FLD) on lettuce

摘要

Free-living diazotrophic?isolates L1?(Bacillus cereus?Frankland) and?Br2 (Bacillus subtilisEhrenberg Cohn)?were evaluated in greenhouse trials for their optimum dose and frequency of application on seedlings of a single lettuce variety (Great Lakes) in a composted pine bark medium. Bacterial?isolates were grown in Burke’s broth and were applied as a drench at four different doses (105, 106, 107?and 108?colony forming units (cfu) ml-1) and at different frequencies of application (1, 7, 14 and 21 days). Two months later, wet weight, dry weight and plant N levels were measured. Lettuce growth and plant N level responded positively to both bacterial isolates inoculated at 106cfuml-1?weekly and fortnightly. Isolate Br2 inoculated at 106cfuml-1?fixed 32.4% plant N when applied weekly and 26.7% when applied every two weeks. Isolate L1, at the same dose, applied weekly or every two weeks, fixed 27.7 and 29.1% of plant N requirement, respectively. The lettuce seedlings responded less well to a higher dose at 108cfu ml-1?of either isolate applied weekly and every two weeks. The response to dosage depended on frequency of application. Doses of 106?cfu ml-1?of both isolates applied weekly or every two weeks had the best effect on lettuce seedling growth and plant N.