Antagonistic effects of vitamin E on transcription activity cyP450 and antioxidant enzyme genes in the liver of mice treated acutely with TCDD

摘要

To investigate the effects of vitamin E on transcription activity of liver CYP 4501A 1 ， CYP 4501A 2 ， CYP4501B1 ， SOD ， GSH-Px ， CAT gene in mice treated acutely with 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin （ TCDD ） . The acute treatment was assigned 3 groups, and the level of both TCDD and vitamin E were 0 and 0, 30 and 0, 30 μg/kg and 100 mg/kg·d, respectively. Livers were collected to analyze transcription activity of CYP 4501A 1, CYP 4501A 2, CYP4501B1, SOD, GSH-Px, CAT gene by Real-time PCR method. The results showed that 1)the level of CPY 4501A 1, 1A 2, 1B 1 in the acute TCDD-treatment were significant higher than the that of control group , vitamin E(100 mg/kg) inhibited the significant increase; 2)there was a decreasing tendency of SOD, GSH-Px mRNA levels and significant decrease of CAT mRNA level after acute treatment with TCDD, the mRNA level of SOD and CAT had significant increase, while the level of GSH-Px had a increasing tendency in the group supplemented vitamin E 100 mg/kg compared to the group treated with TCDD. These results suggested that TCDD can affect or partly affect transcription activity of liver CYP450 genes and antioxidant enzyme gene , in mice, while vitamin E can alleviate or partly alleviate the TCDD effects [ Acta Zoologica Sinica 54(6): 1038–1043, 2008] .