Effect of cysteamine on mRNA expression and activity of H+-K+-ATPase in gastric mucosa of pre- and post-weaning piglets

摘要

18 litters of newborn piglets were divided randomly to control and treatment groups. From 12 days of age, piglets in treatment group were fed starter diet supplemented with 120 mg/kg cysteamine, while the control group received basal diet. Piglets were weaned at 35 days of age (D35) in both groups. 6 piglets from each group were slaughtered on D28 (one week before weaning), D35 (weaning day), D36.5, D38, D42 and D45 (36 h, 72 h, one week and 10 days after weaning), respectively. A semi-quantitative RT-PCR was applied to determine the levels of H⁺-K⁺-ATPase mRNA in gastric tissues. H⁺-K⁺-ATPase activity in gastric tissue homogenate was also determined. The results indicated that: (1) H⁺-K⁺-ATPase mRNA expression and H⁺-K⁺-ATPase activity in control group exhibited a trend of increase from D28 to D45,reaching a peak on D45, but did not show significant age differences( P >0.05);(2) cysteamine increased the level of H⁺-K⁺-ATPase mRNA expression and the differences were significant on D28, D35, D42 and D45, respectively; the H⁺-K⁺-ATPase activity in treatment group markedly increased by 32.3% on D35 and 18.3% on D45（ P 0.05) was observed between H⁺-K⁺-ATPase mRNA expression and H⁺-K⁺-ATPase activity throughout the period of investigation. In present study, no clear developmental pattern was observed both for gastric expression of H⁺-K⁺-ATPase mRNA and H⁺-K⁺-ATPase activity in piglets pre- and post-weaning. Furthermore, neither the expression of H⁺-K⁺-ATPase mRNA nor the H⁺-K⁺-ATPase activity was affected significantly by weaning. However, dietary supplementation of cysteamine improved markedly both the mRNA expression and the activity of H⁺-K⁺-ATPase in piglets pre- and post-weaning [ A cta Zoologica Sinica 51(2): 268–273, 2005].