Effect of Nicotine on Hematology, Lipid Profile and Liver Enzymes in Adult Male Mice ( Mus Musculus )

摘要

This study was designed to observe the effects of nicotine on various hematological parameters, lipid and hepatic profile of adult male mice. A total of 80 mice were divided into two groups; control (n=40) and experimental (n=40). Subcutaneous injections of 1 mg/kg body weight of nicotine were given to the experimental and saline solution was given to the control group. The treatment lasted for 6 weeks after which blood was collected. Using hematological analyzer, hematological parameters were assessed whereas biochemical analysis was performed by chemistry analyzer. Statistical analysis was performed on SPSS. Comparison between the groups was done by T–test. The white blood cells, Platelet, Hematocrit, mean corpuscular volume, cholesterol, triglycerides and low density lipoprotein cholesterol were found significantly higher (p≤0.05) in an experimental group compared to control group while the significant decrease was observed in red blood cell count, hemoglobin, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration, high density lipoprotein cholesterol and HDL–C/LDL–C ratio in the experimental group. Significant increase in the liver enzymes, alanine amino transferase aspartate aminotransferase and alkaline phosphatase were observed, where as total bilirubin (mg/dl), albumin and total protein showed significant decrease in the experimental group. It is concluded that nicotine administration in mice resulted in deleterious effects on various hematological and biochemical parameters