Relationship of in vitro Cervical Mucus Penetration Assay with IgA and IgG Type Antibodies in Cross-bred Cows

摘要

IgG and IgA class antisperm antibodies were detected in cervical mucus of 25 crossbred cows with SperMar test. Sperm penetration through cervical mucus of these cows was also analysed in vitro. Relationship of in vitro CMPT with IgA and IgG type antibodies in cervical mucus was evaluated. Animals were grouped as G-1(>40% IgG, IgA) and G-II ( 40% IgA and IgG were detected in 13 and 17 cows respectively. Among the cows with higher percentage of IgA/IgG, there were only 6 cows (28%) that had higher level of both IgA and IgG (>40%) and low values of CMPT. There were also one (3.8%) and four (15.3%) cows with only > 40% IgA and IgG and low values of CMPT, respectively. It can be concluded that higher percentage of IgG-ASA and IgA-ASA reduced in vitro penetration of spermatozoa through cervical mucus in 44% of the tested cross-bred cows. However, there was not any negative effect of ASA on in vitro CMPT in a group of cows with > 40% IgG/IgA in cervical mucus