Hapten Design and Antibody Generation for Immunoanalysis ofSpirotetramat and Spirotetramat-enol

摘要

Spirotetramat a tetramic acid insecticideis rapidly metabolized or degraded to give spirotetramat-enol; so,common residue definitions include the sum of both compounds. In the present study, two spirotetramat-functionalizedderivatives (haptens) have been designed to generate immunoreagents to these molecules for rapid immunochemical analysis.Haptens have been synthesized with alternative linker tethering sites and, for the first time, high-affinity antibodies have beengenerated with different specificities to these active principles. Two sensitive assays have been developed using the sameantibody in different formats, and by using linker-site heterologous haptens, the selectivity of the final immunoassay could beimproved. A generic immunoassay with sensitivity similar to spirotetramat and spirotetramat-enol and a specific assay ofspirotetramat-enol have been developed. The described antibody and bioconjugates showed great potential for sensitiveimmunosensor development and analysis of this complex analyte.