Isolation and Characterization of Listeria Monocytogenes from Goat Brain

摘要

Caprine listeriosis caused by Listeria monocytogenes is a disease of zoonotic importance. Listeriosis involves central nervous system of goat which is manifested in various clinical signs. A total of 69 brain samples from different breeds of goat collected from the post–mortem cases and slaughter house were examined for the presence of pathogenic Listeria. Twenty one brain samples were found positive for listeria by cultural, biochemical, phenotypic and genotypic characteristics. Among these, nine isolates were characterized as Listeria monocytogenes. The isolates were positive for the hly and actA virulence genes by PCR assay. All the isolates belonged to 4b, 4d and 4e serovar group using multiplex PCR based serotyping. No characteristic pathological lesions of listeriosis have been found in the brain. The isolation of L. monocytogenes is important with regard to food safety and public health, representing a possible link between the animals, environment and human infection